SUNRESIN
Stock Code 300487
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Agarose-Based Chromatography Resins
Agarose-Based Chromatography Resins
Ion Exchange Chromatography – Agarose
Ion Exchange Chromatography – Agarose
Strong Cation
Strong Cation
SP
SP

Seplife® Q HiFlo 90

  • High stability to CIP (cleaning in place) up to 1M NaOH
  • Hydrophilic base matrix ensures low levels of non-specific adsorption and high recovery rate
  • Regulatory Support File (RSF) is available for Seplife® Q HiFlo 90.
  • Seplife® Q HiFlo 90 is a strong anion exchanger agarose resin suitable for intermediate and polishing steps in proteins, mAbs and nucleic acids downstream processing.
    Seplife® Q HiFlo 90 is based on highly cross-linked agarose and has a particle size suitable for high-capacity applications (45-165 micron).
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Products

Properties

Product

Seplife® Q HiFlo 90

Appearance

White to light yellow beads

Type

Strong base anion - Quaternary amine

Matrix

 

Ion exchange capacity (mmol/ml)

0.18-0.25 (Cl-)

pH ligand fully charged

Positively charged at pH<11

Particle size range (μm)

45-165

pH stability

2-12 (operational), 2-14 (CIP)

 

Chemical Stability

Stable in all common aqueous buffers; 1M sodium hydroxide; 8M urea; 6M guanidine hydrochloride; 70% ethanol.

Flow rate* (cm/h)

>150

10% Dynamic binding capacity (mg/ml)**

≥40.0

Shipped as

Slurry in 20% ethanol solution

*Testing conditions: Chromatography column 26mm×300mm; column bed height 20cm; 0.1MPa; temperature 25ºC; mobile phase water.

** Testing conditions: Binding buffer: 50mM Tris-HCl, pH8.0; Elution buffer: 50mM Tris-HCl+1M NaCl, pH 8.0 Sample: BSA; Column 8mm*100mm, room temperature, retention time 2 minute.

Storage

Sealed and stored at 4-30°C (preservation solution is 20% ethanol) in a ventilated, dry and clean place, do not freeze.

Transportation

Avoid sunlight, rain, and heavy pressure during transportation. It is strictly forbidden to transport together with toxic and hazardous materials.

Ordering information

Product Name

 

Product Code

 

Pack Size

 

 

 

 

Seplife® Q HiFlo 90

A2160302

25ml

A2160303

100ml

A2160304

500ml

A2160305

1L

A2160306

5L

A2160307

10L

Production date: See label

Expiry date: 5 years, under proper storage conditions

 

Manufacturer: Sunresin New Materials Co. Ltd.

Add:No. 135, Jinye Rd, Xi'an Hi-tech Industrial Development Zone, Shaanxi, 710076, China

www.seplite.com    www.sunresin.com

E-mail: info.lifescience@sunresin.com

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