Seplife® K602 FF

Instructions

Column packing

Column packing should be done according to standard operating procedures. It is important to ensure that each material is at its working temperature, and when possible, the chromatography media may be degassed before column packing.

Equilibration

Equilibrate the column with an appropriate 2-5 column volume buffer. Ensure the conductivity and pH of the effluent are exactly the same as the buffer.

Sample feeding

1. The sample is prepared in the equilibration buffer; turbid sample should be centrifuged and filtered before loading.

2. Generally, polyphenols bind to the Seplife K602 FF media, and the rest of the components flow through the media. The composition of the polyphenols is related to the type, turbidity, and the ingredients used in the beer.

3. The polyphenols are removed from the resin by NaOH rinsing.

Regeneration

Generally, use 1M NaOH solution to wash more than 10 times the volume of the column. Then wash with the equilibration solution or water until the equilibrium is reached (pH and conductivity).

If there are inactivated proteins or lipids that cannot be washed away during regeneration, they can be removed by cleaning in place (CIP).

Cleaning-in-place (CIP)

1. For proteins bound by ionic bonds, 0.5-1 BV of 2M NaCl can be used to remove them.

2. For precipitated proteins, hydrophobically bound proteins or lipids, first wash with 1 BV of 0.1M NaOH, and then wash with equilibrium buffer solution until the pH is neutral.

3. For proteins and lipids with strong hydrophobic binding, wash with 4-10 BV of 70% ethanol or 30% isopropanol. It should be noted that the concentration of the organic solvent should gradually increase to avoid bubbles.

Storage

Sealed and stored at 4-30°C (preservation solution is 20% ethanol ) in a ventilated, dry and clean place, do not freeze.

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